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高丹 讲师 主要从事微流控芯片及液质联用等技术在生命科学中的基础与应用研究

姓名:高丹

职称:讲师

 

【联系方式】

电话:0755-26036035

Email:gao.dan@sz.tsinghua.edu.cn

 

【个人简历】

2006年9月~2011年6月 北京化工大学与清华大学联合培养 化学工程与技术专业 博士

2011年7月~2013年6月 清华大学深圳研究生院 分析化学专业 博士后

2013年8月至今 清华大学深圳研究生院 讲师

 

【研究领域】

研究方向为生命分析化学,主要从事微流控芯片及液质联用等技术在生命科学中的基础与应用研究,研究领域主要包括微流控芯片、代谢组学、蛋白质组学研究、药物分析等。主要承担了国家自然科学基金面上项目、青年基金、广东省自然科学基金、深圳市基础研究、博士后基金和院青年基金等多个项目,同时作为核心人员参与了本课题组973计划、863计划和重大新药创制等多个重大项目。

 

【论文与专利】

[1] Wenrui Liu, Feng Jin, Dan Gao*, Lu Song, Chao Ding and Hongxia Liu*, Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells, RSC Advances, 2017, 7, 13149–13158.

[2] Yanwei Wang, Dan Gao*, Yongli Chen, Guangnan Hu, Hongxia Liu*, Yuyang Jiang, Development of N,S-doped carbon dots as a novel matrix for the analysis of small molecules by negative ion MALDI-TOF MS, RSC Advances, 2016, 6, 79043-79049.

[3] Xiaojian Shao, Dan Gao*, Yini Wang, Feng Jin, Qin Wu, Hongxia Liu*, Application of metabolomics to investigate the antitumor mechanism of flavopiridol in MCF-7 breast cancer cells, Journal of Chromatography B, 2016, 1025, 40-47.

[4] Xiaojian Shao, Dan Gao*, Yongli Chen, Feng Jin, Guangnan Hu, Yuyang Jiang, Hongxia Liu*, Development of a blood-brain barrier model in a membrane-based microchip for characterization of drug permeability and cytotoxicity for drug screening, Analytical Chimica Acta, 2016, 934, 186-193.

[5] Ti Yang, Dan Gao*, Feng Jin, Yuyang Jiang, Hongxia Liu*, Surface-printed microdot array chips coupled with matrix-assisted laser desorption/ionization mass spectrometry for high-throughput single-cell patterning and phospholipid analysis, Rapid Commun Mass Spectrometry, 2016, 30, 73-79.

[6] Shujuan Wang, Hangrui Bai, Zongwei Cai, Dan Gao, Yuyang Jiang*, Hongxia Liu*, MALDI imaging for the localization of saponins in root tissues and rapid differentiation of three Panax herbs, Electrophoresis, 2016,37, 1956-1966.

[7] Yini Wang, Dan Gao, Bizhu Chu, Chunmei Gao, Deliang Cao, Hongxia Liu*,  Yuyang Jiang, Exposure of CCRF-CEM cells to acridone derivative 8a triggers tumor death via multiple mechanisms, Proteomics 2016, 16, 1177–1190.

[8] Dan Gao1, Yini Wang1, Weiyi Xie, Ti Yang, Yuyang Jiang, Yuewei Guo, Jin Guan*, Hongxia Liu*. Metabolomics Study on the Antitumor Effect of Marine Natural Compound Flexibilide in HCT-116 Colon Cancer Cell Line. Journal of Chromatography B, 2016, 1014, 17-23.

[9] Shujuan Wang, Xiaowu Chen, Hemi Luan, Dan Gao, Shuhai Lin, Zongwei Cai, Jianjun Liu, Hongxia Liu* and Yuyang Jiang*. Matrix-assisted laser desorption/ionization mass spectrometry imaging of cell cultures for the lipidomic analysis of potential lipid markers in human breast cancer invasion. Rapid Communications in Mass Spectrometry, 2016, 30, 1-10.

[10] Hangrui Bai1, Shujuan Wang1, Jianjun Liu, Dan Gao, Yuyang Jiang, Hongxia Liu*, Zongwei Cai*. Localization of ginsenosides in Panax ginseng with different age by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry imaging. Journal of Chromatography B, 2016, 1026, 263-271.

[11] Yongli Chen, Dan Gao*, Hangrui Bai, Hongxia Liu, Shuo Lin, Yuyang Jiang, Carbon Dots and 9AA as a Binary Matrix for the Detection of Small Molecules by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.J. Am. Soc. Mass Spectrom.2016, 27,1227-1235.

[12] Yongli Chen1, Dan Gao1, Hongxia Liu*, Shuo Lin, Yuyang Jiang*. Drug cytotoxicity and signaling pathway analysis with threedimensional tumor spheroids in a microwell-based microfluidic chipfor drug screening. Analytica Chimica Acta, 2015,898, 85-92. (1The two authors contributed equally to this work)

[13] Weiyi Xie, Dan Gao*, Feng Jin, Yuyang Jiang, and Hongxia Liu*. Study of Phospholipids in Single Cells Using an IntegratedMicrofluidic Device Combined with Matrix-Assisted LaserDesorption/Ionization Mass Spectrometry. Analytical Chemistry, 2015, 87, 7052-7059.

[14] Dan Gao,Xiaowu Chen,Xiaomei Yang,Qin Wu,Feng Jin,Hongliang Wen*,Yuyang Jiang,Hongxia Liu*. Stable Isotope Labeling Strategy for CurcuminMetabolite Study in Human Liver Microsomes by LiquidChromatography-Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2015, 26, 686-694.

[15] Xiaowu Chen, Dan Gao, Feng Liu, Xiang Gao, Yufen Zhao, Hongxia Liu, Yuyang Jiang.A novel quantification method for analysis of twenty natural aminoacids in human serum based on N-phosphorylation labeling usingreversed-phase liquid chromatography–tandem mass spectrometry.Anal Chim Acta, 2014, 836, 61-71.

[16] Juntong Wei, Feng Jin, Qin Wu, Yuyang Jiang, Dan Gao*, Hongxia Liu*. Molecular Interaction Study of Flavonoid Derivative 3d with Human Serum Albumin Using Multispectroscopic and Molecular Modeling Approach. Talanta, 2014, 126, 116-121.

[17] Qin Wu1, Dan Gao1, Juntong Wei, Feng Jin, Weiyi Xie, Yuyang Jiang*, Hongxia Liu*. Development of a novel multi-layer microfluidicdevice towards characterization of drugmetabolism and cytotoxicity for drug screening. Chemical Communications, 2014, 50, 2762-2764. (1The two authors contributed equally to this work)

[18] Dan Gao1, Feng Jin1, Hongxia Liu*, Yini Wang,Yuyang Jiang*. Metabonomic study on the antitumor effect offlavonoid derivative 3d in HepG2 cells and itsaction mechanism. Talanta. 2014, 118, 382-388.(1The two authors contributed equally to this work)

[19] Feng Jin1, Dan Gao1, Qin Wu, Feng Liu, Yuzong Chen, Chunyan Tan, Yuyang Jiang. Exploration ofN-(2-aminoethyl)piperidine-4-carboxamideas a potential scaffold for development of VEGFR-2, ERK-2and Abl-1 multikinase inhibitor. Bioorganic & Medicinal Chemistry, 2013, 21, 5694–5706. (1The two authors contributed equally to this work)

[20] Xin Bi, Yibao Jin, Shangfu Li, Dan Gao*, Yuyang Jiang, Hongxia Liu*. Rapid and sensitive determination of fatty acids inedible oil byliquid chromatography–electrospray ionization tandem mass spectrometry. Science China Chemistry, 2013, doi: 10.1007s11426-013-4934-5.

[21] Xin Bi, Yibao Jin, Xiang Gao, Feng Liu, Dan Gao*, Yuyang Jiang, Hongxia Liu*. Investigation of Pokemon-regulated proteins in hepatocellular carcinomausing mass spectrometry-based multiplex quantitative proteomics. European Journal of Mass Spectrometry, 2013, 19(2), 111-121.

[22] Dan Gao, Hongxia Liu, Yuyang Jiang and Jin-Ming Lin*. Recent advances in microfluidics combinedwith mass spectrometry: technologies and applications. Lab on a Chip, 2013, 13, 3309-3322.

[23] Yini Wang1, Dan Gao1, Zhe Chen, Shangfu Li, Chunmei Gao, Deliang Cao, Feng Liu, Hongxia Liu*, Yuyang Jiang*. Acridone Derivative 8a Induces Oxidative Stress-Mediated Apoptosis in CCRF-CEM Leukemia Cells: Application of Metabolomics in Mechanistic Studies of Antitumor Agents, PLoS One, 2013, 8, e63572. (1The two authors contributed equally to this work)

[24] Dan Gao, Hongxia Liu, Jin-Ming Lin*, Yini Wang and Yuyang Jiang*. Characterization of drug permeability in Caco-2 monolayers by mass spectrometry on a membrane-based microfluidic device, Lab on a Chip, 2013, 13, 978-985.

[25] Dan Gao, Hongxia Liu, Yuyang Jiang and Jin-Ming Lin*. Recent development of microfluidic devices in vitro cell culture for cell biology research, Trends in Analytical Chemistry, 2012, 35, 150–164.

[26] Dan Gao, Haifang Li,Niejun Wang, and Jin-Ming Lin*. Evaluation of the Absorption of Methotrexate on Cells and Its Cytotoxicity Assay by Using an Integrated Microfluidic Device Coupled to a Mass Spectrometer, Analytical Chemistry, 2012, 84, 9230-9237.

[27] Feng Jin, Dan Gao, Cunlong Zhang, Feng Liu, Bizhu Chu, Yan Chen, Yu Zong Chen, ChunyanTan*, and Yuyang Jiang*. Exploration of 1-(3-chloro-4-(4-oxo-4H-chromen-2-yl)phenyl)-3-phenylurea derivatives as selective dual inhibitors of Raf1 and JNK1 kinases for anti-tumor treatment, Bioorganic & Medicinal Chemistry, 2012, 21, 824-831.

[28] Feng Jin, Nannan Zhang, Chunyan Tan, Dan Gao, Cunlong Zhang, Feng Liu, Zhe Chen, Chunmei Gao, Hongxia Liu, Shangfu Li, and Yuyang Jiang*. 2'-Chloro-4'-aminoflavone Derivatives Selectively Targeting Hepatocarcinoma Cells: Convenient Synthetic Process, G2/M Cell Cycle Arrest and Apoptosis Triggers, Archiv der Pharmazie, 2012, 345, 525-534.

[29] JiangJiang Liu, Dan Gao, SiFeng Mao, Jin-Ming Lin*. A microfluidic photolithography for controlled encapsulation of single cells inside hydrogel microstructures, Science China Chemistry, 2012, 55, 494-501.

[30] Sifeng Mao, Dan Gao, Wu Liu, Huibin Wei and Jin-Ming Lin*.Imitation of drug metabolism in human liver and cytotoxicity assay using a microfluidic device coupled to mass spectrometric detection, Lab on a Chip, 2012, 12, 219-226.

[31] Dan Gao, Huibin Wei, Guang-Sheng Guo, and Jin-Ming Lin*. Microfluidic Cell Culture and Metabolism Detection with Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometer, Analytical Chemistry, 2010, 82, 5679-5685.

[32] Dan Gao, Jiangjiang Liu, Hui-Bin Wei, Hai-Fang Li, Guang-Sheng Guo, Jin-Ming Lin*. A Microfluidic Approach for Anticancer Drug Analysis Based on Hydrogel Encapsulated Tumor Cells, Analytica Chimica Acta, 2010, 665, 7–14.

[33] Dan Gao, Hai-Fang Li, Guang-Sheng Guo, and Jin-Ming Lin*. Magnetic Bead Based Immunoassay for Enumeration of CD4+ T Lymphocytes on a Microfluidic Device, Talanta, 2010, 82, 528-533.

[34] Huibin Wei, Haifang Li, Dan Gao and Jin-Ming Lin*. Multi-channel Microfluidic Devices Combined with Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry Applied to the Monitoring of Glutamate Release from Neuronal Cells, Analyst, 2010, 135, 2043-2050.

[35] Chuansen Liu, Jiangjiang Liu, Dan Gao, Mingyu Ding and Jin-Ming Lin*. Fabrication of Microwell Arrays Based on Two-dimensional Ordered Polystyrene Microspheres for High-throughput Single-Cell Analysis, Analytical Chemistry, 2010, 82, 9418-9424.

[36] Jiangjiang Liu, Dan Gao, Hai-Fang Li and Jin-Ming Lin*. Controlled Photopolymerization of Hydrogel Microstructures inside Microchannels for Bioassays, Lab on a Chip, 2009, 9, 1301–1305.

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